Thermal Inactivation of Staphylococcal Enterotoxins B and C

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Thermal inactivation of staphylococcal enterotoxins B and C.

Thermal inactivation profiles of staphylococcal enterotoxins B (SEB) and C (SEC) at 80, 100, and 121 C showed that SEC is more resistant than SEB to heat. After 24 h of incubation at 25 C, some reactivation (recovery of serological reactivity) occurred in toxins that had been inactivated by heat. If the toxin was stirred during heating, reactivation did not occur. An examination of the reactiva...

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Staphylococcal Enterotoxins

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Production of staphylococcal enterotoxins A, B, and C under conditions of controlled pH and aeration.

The production of enterotoxins A, B, and C by nine strains of Staphylococcus aureus has been studied under controlled conditions in a fermenter. The strain to strain differences between staphylococci producing a specific enterotoxin were very marked. Increasing aeration in shake flasks improved both growth and production of all extracellular proteins measured other than that of enterotoxin C, t...

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Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.

An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1973

ISSN: 0003-6919

DOI: 10.1128/am.26.6.938-942.1973